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tjp1  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc tjp1
    Warm acupuncture enhances intestinal barrier integrity and suppresses histidine metabolism in CUMS rats. ( A – C ) mRNA expression levels of tight junction–related genes Occludin, <t>Tjp1</t> (ZO-1), and Claudin4 in colonic tissues, determined by RT-qPCR. ( D ) Representative Western blot and quantitative analysis of Occludin, Tjp1, and Claudin4 protein expression in the colon. ( E ) mRNA expression of histidine metabolism–related enzymes HAL and HDC, showing upregulation in CUMS rats and suppression by MMWA intervention. Data are presented as mean ± SD (n = 3–6/group). * p < 0.05, *** p < 0.001 vs Control; # p < 0.05, ## p < 0.01, ### p < 0.001 vs CUMS.
    Tjp1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 97/100, based on 415 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+anti+zo+1+tjp1/ZO-1+Rabbit+mAb/pmc12764300-86-17-20
    Average 97 stars, based on 415 article reviews
    tjp1 - by Bioz Stars, 2026-10
    97/100 stars

    Images

    1) Product Images from "Antidepressant-Like Effects of Mongolian Medical Warm Acupuncture via Remodeling the Gut Microbiota–Metabolite–Barrier Axis in CUMS Rats"

    Article Title: Antidepressant-Like Effects of Mongolian Medical Warm Acupuncture via Remodeling the Gut Microbiota–Metabolite–Barrier Axis in CUMS Rats

    Journal: Neuropsychiatric Disease and Treatment

    doi: 10.2147/NDT.S566386

    Warm acupuncture enhances intestinal barrier integrity and suppresses histidine metabolism in CUMS rats. ( A – C ) mRNA expression levels of tight junction–related genes Occludin, Tjp1 (ZO-1), and Claudin4 in colonic tissues, determined by RT-qPCR. ( D ) Representative Western blot and quantitative analysis of Occludin, Tjp1, and Claudin4 protein expression in the colon. ( E ) mRNA expression of histidine metabolism–related enzymes HAL and HDC, showing upregulation in CUMS rats and suppression by MMWA intervention. Data are presented as mean ± SD (n = 3–6/group). * p < 0.05, *** p < 0.001 vs Control; # p < 0.05, ## p < 0.01, ### p < 0.001 vs CUMS.
    Figure Legend Snippet: Warm acupuncture enhances intestinal barrier integrity and suppresses histidine metabolism in CUMS rats. ( A – C ) mRNA expression levels of tight junction–related genes Occludin, Tjp1 (ZO-1), and Claudin4 in colonic tissues, determined by RT-qPCR. ( D ) Representative Western blot and quantitative analysis of Occludin, Tjp1, and Claudin4 protein expression in the colon. ( E ) mRNA expression of histidine metabolism–related enzymes HAL and HDC, showing upregulation in CUMS rats and suppression by MMWA intervention. Data are presented as mean ± SD (n = 3–6/group). * p < 0.05, *** p < 0.001 vs Control; # p < 0.05, ## p < 0.01, ### p < 0.001 vs CUMS.

    Techniques Used: Expressing, Quantitative RT-PCR, Western Blot, Control

    Related Articles

    other:

    Article Title: Proteomic Analysis of Epithelial to Mesenchymal Transition (EMT) Reveals Cross-talk between SNAIL and HDAC1 Proteins in Breast Cancer Cells
    Article Snippet: Rabbit anti-β-actin (#4970), rabbit anti-GAPDH (#2118), rabbit anti-HDAC1 (#2062), rabbit anti-MARCKS (#5607), rabbit anti-Smad 2/3 (#8685), rabbit antiphospho-Smad2 (Ser465/467)/Smad3 (Ser423/425) (#8828), rabbit anti-Snail (#3879), rabbit anti-phospho-Akt (Ser 473) (#4060), rabbit anti-Akt (#9272), rabbit anti-ZO-1(TJP1) (#8193), rabbit anti-LGALS1 (#12936), rabbit anti-SOD2 (#13194), rabbit anti-β-tubulin (#2128), rabbit anti-integrin β1 (#9699), mouse anti-cdc2 (#9116), rabbit anti-Lyn (#2796), rabbit anti-acetyl-histone H2B (Lys20) (#2571), and horseradish peroxidase-conjugated secondary antibody goat anti-rabbit IgG and anti-mouse IgG were purchased from Cell Signaling (Beverly, MA, USA).

    Article Title: Proteomic Analysis of Epithelial to Mesenchymal Transition (EMT) Reveals Cross-talk between SNAIL and HDAC1 Proteins in Breast Cancer Cells
    Article Snippet: Rabbit anti-β-actin (catalog no. 4970), rabbit anti-GAPDH (catalog no. 2118), rabbit anti-HDAC1 (catalog no. 2062), rabbit anti-MARCKS (catalog no. 5607), rabbit anti-Smad 2/3 (catalog no. 8685), rabbit anti-phospho-Smad2 (Ser-465/467)/Smad3 (Ser-423/425) (catalog no. 8828), rabbit anti-Snail (catalog no. 3879), rabbit anti-phospho-Akt (Ser- 473) (catalog no. 4060), rabbit anti-Akt (catalog no. 9272), rabbit anti-ZO-1(TJP1) (catalog no. 8193), rabbit anti-LGALS1 (catalog no. 12936), rabbit anti-SOD2 (catalog no. 13194), rabbit anti-β-tubulin (catalog no. 2128), rabbit anti-integrin β1 (catalog no. 9699), mouse anti-Cdc2 (catalog no. 9116), rabbit anti-Lyn (catalog no. 2796), rabbit anti-acetyl-histone H2B (Lys20) (catalog no. 2571), and horseradish peroxidase-conjugated secondary antibody goat anti-rabbit IgG and anti-mouse IgG were purchased from Cell Signaling (Beverly, MA).



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    Image Search Results


    Warm acupuncture enhances intestinal barrier integrity and suppresses histidine metabolism in CUMS rats. ( A – C ) mRNA expression levels of tight junction–related genes Occludin, Tjp1 (ZO-1), and Claudin4 in colonic tissues, determined by RT-qPCR. ( D ) Representative Western blot and quantitative analysis of Occludin, Tjp1, and Claudin4 protein expression in the colon. ( E ) mRNA expression of histidine metabolism–related enzymes HAL and HDC, showing upregulation in CUMS rats and suppression by MMWA intervention. Data are presented as mean ± SD (n = 3–6/group). * p < 0.05, *** p < 0.001 vs Control; # p < 0.05, ## p < 0.01, ### p < 0.001 vs CUMS.

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    doi: 10.2147/NDT.S566386

    Figure Lengend Snippet: Warm acupuncture enhances intestinal barrier integrity and suppresses histidine metabolism in CUMS rats. ( A – C ) mRNA expression levels of tight junction–related genes Occludin, Tjp1 (ZO-1), and Claudin4 in colonic tissues, determined by RT-qPCR. ( D ) Representative Western blot and quantitative analysis of Occludin, Tjp1, and Claudin4 protein expression in the colon. ( E ) mRNA expression of histidine metabolism–related enzymes HAL and HDC, showing upregulation in CUMS rats and suppression by MMWA intervention. Data are presented as mean ± SD (n = 3–6/group). * p < 0.05, *** p < 0.001 vs Control; # p < 0.05, ## p < 0.01, ### p < 0.001 vs CUMS.

    Article Snippet: Membranes were blocked and incubated overnight at 4 °C with primary antibodies against Occludin (1:1000, Abcam, ab216327), TJP1 (ZO-1, 1:1000, CST, # 13663), Claudin4 (1:1000, Abcam, ab15104), HAL (1:500, Proteintech, 15756-1-AP), HDC (1:500, Santa Cruz, sc-271213), and β-actin (1:2000, Abcam, ab8226).

    Techniques: Expressing, Quantitative RT-PCR, Western Blot, Control